Journal: bioRxiv
Article Title: Cancer-Associated Mesothelial Cells Drive Immune Escape and Therapy Resistance in Ovarian Cancer
doi: 10.64898/2026.01.07.698232
Figure Lengend Snippet: A) Quantitative PCR (qPCR) analysis of Upk3b and Serpinb2 expression in immortalized mesothelial cells (iMC) stimulated with KPCA-conditioned media and Met5a stimulated with patient D HGSOC media (ptD) or Ov90 ovarian cancer cell line in a time-dependent manner (0-96hrs). B) Western blot analysis of UPK3B and SERPINB2 expression in iMC treated with KPCA-condioned culture medium at 0h, 24h, 48h, 72h and 96h (left panel) and Met5A stimulated with conditioned media from ptD/Ov90 cell-lines versus unstimulated controls at 72h (right panel). C) Immunofluorescence analysis of UPK3B and SERPINB2 in iMC and Met5A comparing conditions stimulated with either KPCA-conditioned media or ptD/Ov90 media versus unstimulated controls at 72 h. D) Immunofluorescence staining for ACTA2 and COL4A1 in both primary MCs and immortalized MCs (iMCs) after 96 hours of exposure to KPCA-conditioned media
Article Snippet: The Met5a cell line was purchased from ATCC and cultured in DMEM medium supplemented with 5% FBS.
Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Immunofluorescence, Staining